色谱柱极性_范文大全网

范文一：色谱柱极性

色谱柱极性

一、非极性1、100%Dimethyl polysiloxane，100%聚二甲基硅氧烷，商品名：AC1，OV-101，OV-1，DB-1，SE-30，HP-1，RTX-1，BP-1二、弱极性2、5%Phenyl dimethyl polysiloxane, 5%二苯基(95%)二甲基聚硅氧烷，商品名：AC5，SE-52，3、5% Phenyl 1%vinyl dimethyl polysiloxane，5%二苯基1%乙烯基(94%)二甲基聚硅氧烷，商品名：OV-5，DB-5，SE-54，HP-5，RTX-5，BP>>>

按相似相容原理，一般极性物质用极性色谱柱进行分离，但也不一定，有些极性物质在飞极性柱子或中极性柱子上照样良好分离，峰型不错。也要考虑沸点，化合物的性质等因素。有的极性化合物在极性柱子上甚至无法出峰。硝基苯有人用三氟丙基甲基硅油柱，SE-30等柱分离。

硝基苯可以用ZB-1701的柱子分离，效果很好的

甲醇做溶剂，ECD检测（使用温度最好在310以上），灵敏度很好

不一定需要极性色谱柱。

范文二：非极性色谱柱与极性色谱柱的区别

柱子的极性取决于固定相的极性我们常用柱子的极性如下:一、非极性 1、100%Dimethyl polysiloxane，100%聚二甲基硅氧烷，商品名：AC1，OV-101，OV-1，DB-1，SE-30，HP-1，RTX-1，BP-1二、弱极性2、5%Phenyl dimethyl polysiloxane, 5%二苯基(95%)二甲基聚硅氧烷，商品名：AC5，SE-52，3、5% Phenyl 1%vinyl dimethyl polysiloxane，5%二苯基1%乙烯基(94%)二甲基聚硅氧烷，商品名：OV-5，DB-5，SE-54，HP-5，RTX-5，BP-5注：2、3常无严格区分，通常混称。三、中等极性4、50%Phenyl dimethyl polysiloxane, 50%二苯基(50%)二甲基聚硅氧烷，商品名：OV-17，HP-50，RTX-505、14%Cyanopropyl phenyl polysiloxane, 14%氰丙基苯基(其中7%氰丙基7%苯基)(86%)二甲基聚硅氧烷，商品名：AC10，OV-1701，DB-1701，RTX-17016、50% Cyanopropyl phenyl polysiloxane，50%氰丙基苯基(其中25%氰丙基25%苯基)(50%)二甲基聚硅氧烷，商品名：AC225，OV-225，BP-225，DB-225，HP-225，RTX-225四、强极性7、polyethylene glycol,聚乙二醇，商品名：AC20，PEG20M，HP-INNOWAX(FFAP是其与2-硝基对苯二甲酸的反应产物)

范文三：极性色谱柱的区别

As the frequency of polar labora-

tory expeditions increases andthe range of exploration expands,so has our line of polyethyleneglycol (PEG) capillary columns.Added to the already powerful lineupof HP-Wax, HP-INNOWax, HP-FFAP,and HP-20M capillaries is the newfamily of HP-Basic Wax columns.Each of these five column types isavailable in a variety of diameters,lengths and film thickness includingHP-INNOWax, HP-Wax, HP-BasicWax, HP-FFAP and HP-20M.Even though each phase is based onthe polyethylene glycol polymer,

strict control of the cross-linking anddeactivation processes results in avariety of unique phase characteris-tics to meet the varying analysisneeds of your laboratory.

A Full Family of Polar PEGColumnsFrom Agilent

Product Brief

Columns and Supplies

Versatile HP-INNOWax

wide range of analytes and solvents,Since its introduction, the HP-long lifetime, and low-bleed charac-INNOWax column has become theteristics, the HP-INNOWax columnsworkhorse of the PEG columns.are used in a wide range of Because of a compatibility with aapplications.

Industrial Solvents

1.Pentane

Column:

HP-Wax, 30 m x 0.25 mm x 0.25 μm 2.Methyl formate(Part number 19091X-133)

3.AcetoneCarrier:Hydrogen, 40 cm/sec4.Ethyl acetate

Oven:35°C (2.5 min) 10°C/min to 85°C5.Methyl ethyl ketoneInjection:Split (100:1), 0.5 μl6.Methanol

Detector:FID300°C

7.2-Methyl-2-propanol8.Methylene chloride9.Benzene10.Ethanol12

11.2-Butanol1512.Toluene9

13.n-Propanol14.Ethyl benzene15.p-Xylene16.m-Xylene11

17.1-Butanol5,6

18.

o-Xylene

801234567 min

The HP-INNOWax column has thehighest upper temperature limit ofany of the wax phases. With a maxi-mum upper temperature limit for programmed operation of 270°C forstandard dimensions and 250°C for0.53 mm columns, the HP-INNOWaxcolumn is ideal for the analysis ofhigh boiling point analytes that maybe difficult or impossible to do onother “Wax” columns. For example,free fatty acids to C24 can be ana-lyzed with this column. The versatili-ty of the HP-INNOWax column is sec-ond to none. The superior inertnessproduces sharp, reproducible peaksfor both basic and acidic compoundswithout requiring regeneration of thecolumn with injections of acids orbases.

Aldehydes and Acids

1.Butanal

10.iso-Butyric acid2.2-Methyl butanal11.Butyric acid3.Pentanal12.iso-Valeric acid4.Hexanal13.Valeric acid5.Heptanal14.Hexanoic acid6.Octanal15.Heptanoic acid7.Acetic acid16.Octanoic acid8.Decanal

17.

Decanoic acid

Propanoic acid

9250°C

101160°C10°C/

min121 min131617

131415

456

805101120 min

Column:

HP-INNOWax

30 m x 0.32 mm x 0.5 μm (Part number 19091N-213)

Carrier:Helium, 40 cm/sec, 11.7 psi (60°C),

2.5 ml/min constant flow

Oven:60°C (1 min) to 250°C at 10°C/minInjection:Split (40:1), 0.5 μl, inlet at 250°CDetector:FID 275°C

PEGColumns for Petrochemical ApplicationsReformate Gasoline Analysis

1.MonaromaticsA

2.Benzene3

3.Toluene4.Ethylbenzene6

5.m-xylene2

6.o-xylene

C9 plus aromatics

5101520 min

2345 min

Column:HP-Wax, 30 m x 0.32 mm x 0.5 μm

Column:

HP-Wax, 10 m x 0.1 mm x 0.2 μm(Part number 19091X-213)

(Part number 19091X-241)

Carrier:Helium, 6.4 psi, constant pressureCarrier:Helium, 37.3 psi, constant pressureOven:60°C (4 min) 10°C/min to 140°C

Oven:60°C (1 min) 36.7°C/min to 140°C

(0 min) 140–200°C (4 min) at 15°C/min

(0 min) 140–200°C (2 min) at 55.1°C/min

Injection:250°C, 0.5 μl, Split 200/1Injection:250°C, 0.1 μl, Split 800/1Detector:275°C, FIDDetector:275°C, FID

Impurities in p-Xylene:ASTMD3798

Column:

HP-INNOWax, 60 m x 0.32 mm x 0.5 μm (Part number 19091N-216)

Carrier:He, 32 cm/sec, 19.9 psi (60°C), 2.5 ml/min constant flowOven:60°C (1 min) to 92°C (4.5 min) at 4°C/min,

to 220°C (5 min) at 20°C/min

Injection:Split (split ratio 100:1), 0.5 μl Inlet 220°CGC System:Agilent GC/FID (270°C)5

Sample:Neat, 99%+1.Non-aromatic hydrocarbon2.Toluene4

3.Ethylbenzene4.p-Xylene5.m-Xylene5

o-Xylene

101112 min

1015 min

HP-INNOWax temperature range:40°C to 260°C;

0.53 mm id columns:40°C to 240°C

HP-INNOWax

Similar Phases

Stabilwax, Supelcowax-10,CPWAX 52 CB, DB-WAXetr

Applications

Alcohols, Free Acids,Aromatics, Essential Oils,Solvents

The HP-INNOWax columns have thelowest bleed of any of the Wax phas-es. This results in lower detectornoise so you can detect and quanti-tate lower levels of compounds. Thisphase is also a good choice for use inmass spectrometry where columnbleed can reduce the accuracy ofpeak identification.

The combination of a highly deacti-vated glass surface with the cross-linked and surface bonding results ina column that is compatible with awide range of polar compounds. Thismakes the HP-INNOWax column theideal choice when developing newmethods. The HP-INNOWax family ofcolumns have been used successfullyin a variety of different industries andapplications including petrochemical,solvent analysis, food and flavors,and beverage analysis.

PEGColumns for Petrochemical ApplicationsImpurities in Styrene: ASTMD5135

1.NonaromaticsColumn:

HP-INNOWax, 30 m x 0.32 mm x 9

2.Ethylbenzene0.5 μm (Part number 19091N-213)

3.p-XyleneOven:80°C (5 min), 10°C/min to 150°C

4.m-Xylene

(2 min)

5.IsopropylbenzeneCarrier:Helium 8.6 psi (28 cm/sec),

6.o-Xylene

constant flow, 1.5 ml/min

7.n-PropylbenzeneInjection:0.5 μl split 40:1, 230°C8.p/m-EthyltolueneGC System:Agilent GC/FID 240°C

9.Styrene

10.

a-Methylstyrene (366 ppm)

11.Phenylacetylene

(58 ppm)

212.?-Methylstyrene4

13.Benzaldehyde11

7812

2.5

7.5

12.5 min

Impurities in Ethylbenzene:ASTMD5060

1.Hydrocarbon4

2.Benzene3.Toluene4.Ethylbenzene5.p-Xylene6.m-Xylene4

7.Cumene8.o-Xylene

9.Propylbenzene5

10.

Styrene

1011min

Column:

HP-INNOWax, 60 m x 0.32 mm x 0.5 μm1

910

(Part number 19091N-216)

Carrier:He, 32 cm/sec, 19.9 psi (60°C),

2.5 ml/min constant flow

510

15 min

Oven:60°C (1 min) to 92°C (4.5 min) at4°C/min,to 220°C (5 min) at 20°C/min

Injection:Split (split ratio 100:1), 0.5 μl Inlet 220°CGC System:Agilent GC/FID 270°CSample:Neat, 99%+

Low Operating TemperatureHP-WAX

The HP-Wax column has the lowestoperating temperature of any of theWax phases. The proprietary cross-linking lowers the usable tempera-ture of the stationary phase to 20°Ccompared with 40°C for other Waxphases making this phase ideal forlow boiling point compounds.If your application is purge and trapor headspace, the HP-Wax columncan be used with cryogenic coolingfor cold trapping analytes. The HP-Wax column is designed to match thepolarity of competitive Wax columnsmaking substitution easy. The HP-Wax phase is the closest in polarityto the HP-20M so you can transferyour methods to a cross-linked phase without lengthy method development.

Solvent Analysis

HP-INNOWax, HP-Wax Comparison

1.2.3.4.5.6.7.8.9.10.11.12.13.14.15.16.17.18.

Pentane

Methyl formateAcetoneEthyl acetate

Methyl ethyl ketoneMethanol

2-Methyl-2-propanolMethylene chlorideBenzeneEthanol2-ButanolToluenen-PropanolEthyl benzene

HP-INNOWax,p-Xylene

30 m x 0.25 mm x m-Xylene

0.25 μm1-Butanol

o-Xylene

PEGColumns for Petrochemical/Chemical ApplicationsImpurities in Styrene

Gas

Chromatograph:6890 Series GCColumn:HP-Wax, 10 m x 0.10 mm, 0.2 μm

(Part number 19091X-241)

Injection:0.1 μl split 300, 230°COven:80°C (1.4 min), 33.8°C/min

to 150°C (0.5 min)

Carrier:Helium 46.4 psi (48 cm/sec)

Constant flow, 0.5 ml/min

Detector:FID, 240°C

3 min12

2.3.104.

5.6.7.8.9.10.11.12.13.

NonaromaticsEthylbenzenep-Xylenem-Xylene

Isopropylbenzeneo-Xylene

n-Propylbenzenep/m-EthyltolueneStyrene

a-Methylstyrene (366 ppm)Phenylacetylene (58 ppm)b-MethylstyreneBenzaldehyde

Carrier:Oven:Injection:GC System:Hydrogen, 40 cm/sec, constant pressure35°C (2.5 min), 10°C/min to 85°C250°C, Split (100:1), 0.5 μlAgilent GC/FID, 300°C

9, 10

5, 64

14151617

1211

108

151416

HP-Wax,

30 m x 0.25 mm x 0.25 μm

4567

01234567 min

PEGColumns for Petrochemical/Chemical ApplicationsSubstituted Aromatics HP-Wax Column

1.Hexane10.tert-ButylbenzeneColumn:

HP-Wax, 60 m x 0.32 mm x 0.5 μm 2.Benzene11.Isobutylbenzene(Part number 19091X-216)3.Toluene12.Mesitylene

GC System:Agilent GC/FID, 300°C

4.Ethylbenzene13.sec-ButylbenzeneInjection:Split, 250:1, 0.5 μl injection, 250°C

5.1,4-Xylene14.Styrene

Concentration

6.1,3-Xylene15.1,2,4-Trimethylbenzeneof analytes:500 μg/ml in hexane7.Cumene16.Butylbenzene

Carrier:Hydrogen at 40 cm/sec constant

8.1,2-Xylene17.1,4-Diisopropylbenzenepressure

Propylbenzene

18.

trans-?-Methylstyrene

Oven:50°C for 1 min; 50–75 at 4°C/min,

175–100°C at 10°C/min, (hold 4 min) 100–150°C at 10°C/min

516

91112

1015

024681012141618 min

Solvents in Paint Thinner

HP-Wax,

10 m x 100 μm x 0.2 μm23

235

(Part number 19091X-241)4

1.Propylene oxide60°C (1.5 min)60°C (0.56 min)2.Acetone30°C/min80°C/min

3.Methanol

150°C (2 min)150°C (0.1 min)4.Methylene chloride1000/1, 0.1 μl1000/1, 0.1 μl5.Toluene27.5 psi33.7 psi6.C8 aromatics(0.2 ml/min)(0.6 ml/min)7.

4-hydroxy-4

27 cm/sec

69 cm/sec

methyl-2-pentanone

767

4 min

0.5

1.5 min

Temperature range:20°C to 250°C;

Series 530-μm and thick-filmcolumns: 20°C to 230°C

HP-Wax

Similar Phases

DB-Wax, Rtx-wax,Carbowax 20M

Applications

Alcohols, Aromatics, Essential Oils, Solvents

The HP-Wax columns are available in100 μm diameters for fast analysis.The 100 μm columns have the samephase chemistry as the standard

dimension HP-Wax columns so trans-lating methods to the smaller dimen-sions is simple. If you are using theHP-INNOWax columns, you can stilltranslate your methods to the 100 μmHP-Wax columns. Most methodstranslate directly without the needfor time consuming method develop-ment. Faster analysis means moreproductivity for your lab.

The HP-Wax columns are cross-linked and bonded to ensure long life-time. The columns can be solventrinsed to extend the column lifetimeand reduce costs. Although the HP-Wax columns are used for the sameapplication areas as the HP-INNOWaxcolumns, the HP-Wax has slightly dif-ferent selectivity for some com-pounds like alcohols (see compara-tive chromatogram on page 4). Ifsome analytes are difficult to sepa-rate on the HP-INNOWax columnsand you need the polarity of a Waxcolumn, try the HP-Wax column.

HP-FFAP Columns for FreeFatty Acid Analyses

The HP-FFAP columns are designedprimarily for the analysis of organicacids, free fatty acids or samples thatrequire quantitation of acidic impuri-ties. The stationary phase is modifiedwith acid to provide a very inert col-umn that can accommodate thedemanding analysis of acids dis-solved in water. Free fatty acids upto C24 can be analyzed without cost-ly and time consuming derivatization.If you want to analyze the derivatizedfree acids, the FFAP column is agood choice to resolve the deriva-tized saturated and unsaturated acidsto C24.

The HP-FFAP is cross-linked andbonded to resist the damage that canoccur when injecting water basedsamples and can be used at operatingtemperatures of 60°C to 240°C. Thesecolumns do not require several injec-tions of acids (priming) before accu-rate quantitation is possible so accu-rate results are obtained quickly. Thecolumns are also solvent rinsable forlong column lifetimes.

HP PEGColumns for Foods and Flavors ApplicationsFree fatty acids (C2–C7)

Injection:Column:Carrier:Oven:

GC System:

0.5 μl, split 20/1, 260°C

HP-FFAP, 25 m 0.32 mm 0.5 μm (Part number 19091F-112)Helium @ 10.8 psi (42 cm/sec)

80°C (1 min), to 120°C (20°C/min), then to 205°C (6.13°C/min), 205°C (2 min)Agilent GC/FID, 260°C

321

246810 min

Perfume Calibration Mix

1.2.3.4.5.6.7.8.9.

a-Pineneb-Pinened-LimoneneCitronellalMenthol

Benzyl acetateCitronellyl acetateGeraniol

Hydroxy citronellal

10.11.12.13.14.15.16.17.18.

AnisaldehydeMethyl cinnamateEthyl cinnamateEugenolJasmonal

Diethyl phthalateCoumarinVanillin

Benzyl benzoate5

123

910

Column:

HP-20M (Carbowax 20 M) 50 m x 0.32 mm x 0.3 μm(Part number 19091W-015)

Carrier:Hydrogen, 64 cm/secOven:Temperature program listedInjection:Split, 210°CDetector:FID, 230°C210°C

75°C

1112

1516

5°C/min

35 min

PEGColumns for Foods and Flavors ApplicationsDerivatized Olive Oil (FAMEs) on Two Different Columns

1.C16:02.C16:11

FFAP

3.C18:0Column:HP-FFAP, 25 m x 0.32 mm x 0.5 μm

4.C18:1(Part number 19091F-112)

5.C18:2Carrier:Helium @ 25.7 psi (85 cm/sec)6.C18:35Oven:150°C (1 min) to 230°C (1 min) at

7.C20:02.5°C/min

8.C22:09.

C24:023

1520

30 min

INNOWax

Column:HP-INNOWax, 30 m x 0.25 mm x 0.25 μm

(Part number 19091N-133)

Carrier:Helium @ 46.6 psi (75 cm/sec)5

Oven:

150°C (1 min) to 230°C (1 min) at

2.9°C/min

25 min

Common Parameters

GC:6890Liner:Deactivated with glass wool plug Sample:Greek Olive Oil (Derivatized)

Detector Temp.:260°Cin Methylene Chloride

Hydrogen Flow:40 ml/minSplit Ratio:20/1Air Flow:450 ml/min

Injector Temp.:260°CMakeup Flow:45 ml/min (Nitrogen) Injection Size:1.5 μl

Constant Flow Mode

Jasmine Extract

HP-Wax, 60 m x 250 μm x 0.25 μm(Part number 19091X-136)90 min run

60°C to 248°C (30 min) at 1.5°C/minHelium 1.3 ml/min constant flow1 μl sample, split (50:1)Agilent 6890 Series GC

0102030405060708090 min

HP-FFAP

Similar Phases

DB-FFAP, Stabilwax-DA, OV-351, CPWAX58CB,Nukol

Applications

Acids, Alcohols, Aldehydes, Acrylates, Ketones, Nitriles

Temperature Range

60°C to 240°C

Ethoxyethanol HP-FFAPColumn

1.Ethylene oxide7.Hydroxy acetate2.Ethyl formate8.Acetic acid3.Ethyl alcohol9.Formic acid4.Water10.Ethylene glycol/ 5.2-Ethoxyethanolmonoformate6.

2-Ethoxyethyl acetate11.Ethylene glycol/

monoacetate

TIC(MSD)1024

5611137

20 min

210°C100°C (1 min)10°C/min

60°C5°C/min

1 minColumn:

HP-FFAP, (Cross-linked polyethylene glycol)30 m x 0.53 mm x 1.0 μm(Part no. 19095F-123)

Carrier:Helium at 10 ml/minOven:Temperature program listed belowInjection:Split (10:1), 220°CDetector:MSD (280°C)

HP-Basic Wax

Similar Phases

CAM, Carbowax Amine, Stabilwax-DB, CP-51 WAXfor amines, CP-WAXfor amines and diamines

Applications

Amines and other basic compounds

Temperature Range isothermal/temperature programmed

40°C to 240/260°C

40°C to 220/240°C for 0.53 mm id

HP-Basic Wax for Amines andOther Basic Compounds

The most recent addition to the

Agilent line of PEG capillary columnsis the HP-Basic Wax column. Thisphase has been specifically devel-oped for the analysis of amines andother basic compounds. Peak shapesfor amines show excellent symmetryfrom the very first injection so thereis no need to ‘‘prime’’ the columnwith sample before quantitativeresults can be obtained.Free Fatty Acids

240°C1.Acetic120°C 10°C/min

2.Propionic1 min

3.Butyric4.Valeric1

5.Caproic6.Heptanoic357.

Octanoic7

10 minColumn:

HP-FFAP 30 m x 0.53 mm x 1.0 μm (Part number 19095F-123)

Carrier:Helium, 49 cm/sec, 6 ml/min

constant flow

Oven:120°C (1 min) to 240°C at 10°C/minInjection:Split (15:1), 1 μl, inlet = 240°CDetector:FID (265°C)

Sample:1 to 2% acids in acetone

The HP-Basic Wax column can beinjection and the performance of theused from 60°C to 260°C; one of thecolumn does not deteriorate evenhighest in the industry so you canafter many injections. The columnextend the range of compounds youcan be solvent rinsed (water andcan analyze. Best of all, accuratemethane are not recommended) toquantitation is possible from the firstextend the lifetime of the column.

PEGColumns for Foods and Flavors Applications

Analysis of Amines

Column:

HP-Basic Wax, 30 m x 0.32 mm x 0.25 μm(Part number 19091N-613)

Test Mixture

Carrier:Hydrogen, 6.0 psi(0.5 mg/ml in Hexane)Oven:120°C

1.n-Octylamine6.n-HeptadecaneInjector:250°C, Split ratio: 100:12.n-Nonylamine7.TrihexylamineDetector:250°C, FID

3.n-Decylamine8.n-octyldecane

4.Benzylamine

9.2,6-Dimethylaniline5.Dicyclohexylamine

10.2,4-Dimethylaniline

024681012141618 min

Flavors/Fragrance Standard Mix

1.1-Limonene11.

Benzyl aldehyde20.Phenyl propyl acetate29.Heliotropine2.cis-3-Hexenal12.Linalool21.Maltol

30.Helional3.Prenyl acetate

13.Octanol22.Cyclamen aldehyde31.Idole

4.cis-3-Hexenyl acetate14.Menthol

23.Cinnamic aldehyde32.Methyl naphthyl ketone5.Rose oxide15.Citronellyl acetate24.Isopropyl myristate33.Musk xylol6.Hexenol16.alpha-Terpineol25.Unknown

34.Vanillin

7.Nonanal

17.Benzyl acetate26.Dimethyl anthranilate35.

Ethylene brassylate8.p-Methyl cresol18.Citronellol27.p-Cresol(Musk T)9.Decanal19.

Geraniol

28.

Eugenol10.

Camphor8

3224, 2535

319

1215

410530

1318

1433

1621267

2729

051015202530 min

Column:HP-INNOWax, 30 m x 0.25 mm x 0.25 μm (Part number 19091N-133)Carrier:Helium, 30 cm/sec, 0.9 ml/min, Constant FlowOven:80°C (1 min), 5°C/min to 250°C (2 min)Injection:0.2 μl, On-columnDetector:MSD, 280°C

Sample:

500 ppm in ethanol

PEGColumns for Foods and Flavors ApplicationsVanillin Mix

1.Polyethylene glycol2.Maltol3.Ethyl maltol4.Ethyl vanillin5.Vanillin

System:Column:

Agilent GC/FID, 300°CHP-INNOWax, 30 m x 0.25 mm x 0.25 μm(Part no. 19091N-133)

Carrier:He, 20 cm/sec (100°C),

2.5 ml/min constantflow

Oven:60°C (0 min) to 258°C

(10 min) at 3°C/min

Injection:Split (split ratio 100:1),

0.2 μl, inlet 270°C

Peppermint Oil

1.2.3.4.

LimoneneCineole MenthoneMenthofuran

250°C310°C/min

456

5.6.7.8.7

d-sio-MenthoneMenthyl acetateMentholGermacrene

60°C1 min

10203040506070 min

0Column:

16 min

Brandy

HP 5890 Series IIwith EPCand FID

1.2.3.4.5.6.7.

AcetaldehydeEthyl acetateMethanol35°CEthanol(5 min)n-Propanoliso-Propanol

2-Methyl-1-butanol or 3-Methyl-1-butanol8.Acetic acid

Column:

150°C5°C/min

250°C (2 min)20°C/min

HP-INNOWax (cross-linked PEG)30 m x 0.32 mm x 0.5 μm(Part number 19091N-213)

Carrier:Helium, 40 cm/sec, 11.7 psi (60°C),

2.5 ml/min constant flow

Oven:60°C (1 min) to 250°C at 10°C/minInjection:Split (60:1), 0.5 μl, inlet at 220°CDetector:FID (275°C)Sample:Neat

213

HP-INNOWax, 30 m x 0.25 mm x 0.25 μm

(Part number 19091N-133)

Carrier:He, 30 cm/sec, 0.9 ml/min

constant flow

Injection:Split (10:1), 0.5 ml, inlet 250°CGC System:Agilent GC/MSD (interface 280°C)

01020min

Wine, Cabernet

HP 5890 Series IIwith EPCand FID

GC System:Agilent GC/FID

Automated Sampler:7673 Automated SamplerColumn:HP-INNOWax, 30 m x 0.25 mm x 0.25

μm (Part number 19091N-133)

35°C(5 min)

150°C5°C/min

250°C (2 min)20°C/min

1.2.3.4.5.6.7.

AcetaldehydeEthyl acetateMethanolEthanoln-Propanoliso-Propanol

2-Methyl-1-butanol or 3-Methyl-1-butanol8.Acetic acid

Column:

HP-INNOWax, 30 m x 0.25 mm x 0.25 μm (Part number 19091N-133)

Carrier:He, 33 cm/sec, 15.5 psi (35°C), 1.5 ml/min constant

flow

Oven:35°C (5 min) to 150°C at 5°C/min, to 250°C (2 min) at

20°C/min

Injection:Split (split ratio 25:1), 1 μl, inlet temp at 220°CDetector:FID (280°C)Sample:Neat

321

102030 min

HP-20M

Similar Phases

Carbowax 20M, 007-CW, BP-20

PEGColumns for Paramaceuticals Applications

Alcohols, Adehydes, and Ketones

Calibration standards1.Acetaldehyde2.Acetone 3.Methanol

4.Isopropanol5.Ethanol

6.n-Propanol IS4

Analysis of Ethylene Oxide in aPharmaceutical Formulation

arbs24

22ethylene oxide2018161412

Applications

Alcohols, Free Acids, Ethers, Glycols

acetonitrile

Nonbonded HP-20M Columns

The HP-20M is the nonbonded waxphase that was designed to supportestablished methods that require thepolarity be identical to Carbowax20M. The columns have a tempera-ture range of 60°C to 220°C.

1Column:

2Inlet:Column:

3456 min

HP-INNOWax, 15 m x 0.25 mm x 0.5 μm (Part number 19091N-231)

Detector:FID, 275°C

Carrier:Helium, 1.2 ml/min, Constant FlowOven:40°C (4 min), 10°C/min to 60°C;

25°C/min to 110°C

Injection:0.5 μl, 250°C, Split 250:1

Split/10:1, 1 μl, 250°CHP-WAX, 60 m x 0.53 mm i.d. x 1 μm (Part number19095X-126)

Carrier Gas:HydrogenHead Pressure:25 kPa at 50°C–constant

flow mode

Flow:5.2 ml/min

Oven Temperature:50°C, 5°C/min to 75°C,

25°C/min to 200°C

GC System:Agilent GC/FID, 250°C

Volatile Hydrocarbons and Ketones in Blood

2.3.4.5.6.

Methyl ethyl ketoneBenzene

Methyl isobutyl ketoneToluene

Methyl n-butyl ketoneEthylbenzene

7.8.9.10.11.

p-Xylenem-Xylene1-Butanolo-XyleneStyrene

Organic Volatile Impurities in Pharmaceuticals

1.2.3.4.5.

Methylene chlorideChloroformBenzene

Trichloroethylene1,4-Dioxane

0Headspace sampler:Column:Inlet:

GC System:

5101520 min

6 min

Agilent 7694

HP-INNOWax, 30 m x 0.53 mm x 1.0 μm (Part number 19095N-123)Split/2:1 (180°C)Agilent GC/FID 220°C

EquipmentColumn:

GC Conditions

Oven temperature:40°C (10 min), 5°C/min to 110°CCarrier gas flow rate:3.4 ml/min (25 cm/sec), constant flow

Headspace ConditionsOven temperature:90°CValve and loop temp.:92°CTransfer line temp.:110°CIncubation time:30 minShaking:HighInjection timing:0.5 min pres; 0.02 min vent; 1.0 min injectVial pressure:10.8 psiLoop size:1 mlSample:1.0 ml blood and 100 μl internal standard (0.01% n-butanol in deionized water) to each 22 ml headspace vial.

HP-INNOWax, 30 m x 0.53 mm x 1.0 μm

(Part number 19095N-123)

Detector:FID, 300°C

Sample introduction:Agilent 7694 Headspace SamplerExperimentalCarrier gas:Oven:Injection:Sample:

He, 40 cm/sec, constant flow

50°C (hold 3.5 min) to 60°C at 10°C/min, to 160°C at 40°C/min

Splitless, 1 μl sample volume, 175°C

100 mg pharmaceutical with 50 ppm CHCl3; 100 ppm remaining components

Headspace Sampler ConditionsSetpoint:Oven–80°CTransfer line–110°CLoop–90°CTime events (min):Vent time–0.50Loop equilibrium time–0.05Loop fill time–0.15

GC cycle time–10Pressurize time–0.20

Vial equilibrium time–10 to 60 min with vigorous agitation

范文四：气相色谱中强极性柱、中极性柱、弱极性柱的差别[精品]

一、非极性

1、100%Dimethyl polysiloxane，100%二甲基聚硅氧烷，商品名:AC1，OV-101，OV-1，DB-1，SE-30，HP-1，RTX-1，BP-1

二、弱极性

2、5%Phenyl dimethyl polysiloxane, 5%二苯基(95%)二甲基聚硅氧烷，商品名:AC5，SE-52，

3、5% Phenyl 1%vinyl dimethyl polysiloxane，5%二苯基1%乙烯基(94%)二甲基聚硅氧烷，商品名:OV-5，DB-5，SE-54，HP-5，RTX-5，BP-5

注:2、3常无严格区分，通常混称。

三、中等极性

4、50%Phenyl dimethyl polysiloxane, 50%二苯基(50%)二甲基聚硅氧烷，商品名:OV-17，HP-50，RTX-50

5、14%Cyanopropyl phenyl polysiloxane, 14%氰丙基苯基(其中7%氰丙基7%苯基)(86%)二甲基聚硅氧烷，商品名:AC10，OV-1701，DB-1701，RTX-1701

6、50% Cyanopropyl phenyl polysiloxane，50%氰丙基苯基(其中25%氰丙基25%苯基)(50%)二甲基聚硅氧烷，商品名:AC225，OV-225，BP-225，DB-225，HP-225，RTX-225

四、强极性

7、polyethylene glycol,聚乙二醇，商品名:AC20，PEG20M，HP-INNOWAX(FFAP是其与2-硝基对苯二甲酸的反应产物) 常用毛细管色谱柱对应表 SE-30、OV-1，化学组成:100%甲基聚硅氧烷(胶体)，所属极性:非极性，适用范围:碳氢化合物、农药、酚、胺，对照牌号:DB-1、BP-1、007-1、SPB-1 、RSL-150、CPSRL-5 、HP-1.

OV-101,化学组成:100%甲基聚硅氧烷(流体),所属极性:非极性 ,适用范围:氨基酸、碳氢化合物、药物胺 ,对照牌号:HP-100、SP-2100 SE-52、SE-54,化学组成:5%苯基聚硅氧烷、1%乙烯基 5%苯基甲基聚硅氧烷 ,所属极性:弱极性 ,适用范围:多核芳烃、酚、酯、碳氢化合物、药物胺,对照牌号:DB-5 、BP-5、SPB-5、007-2 、OV-73、CPSIL-8、RSL-120 、HP-5.

OV-1701,化学组成:7%氰丙基、7%苯基甲基聚硅氧烷,所属极性:中极性,适用范围:药物、醇、酯、硝基苯类、除莠剂,对照牌号:BP-10、RSL-1701、DB-1701、HP-1701、CPISL-19.

OV-17,化学组成:50%苯基 50%甲基聚硅氧烷,所属极性:中极性,适用范围:药物、农药,对照牌号:DB-17、HP-17、007-17、SP-2250、RSL-300. OV35,化学组成:35%苯基 65%二甲基聚硅氧烷,所属极性:中极性.

OV-225,化学组成:25%氰丙基、25%苯基、甲基聚硅氧烷,所属极性:中极性,适用范围:脂肪酸甲脂、碳水化合物、中行固醇,对照牌号:DB-225、HP-225、BP-225、CPSIL-43、RSL-500.

OV-275,化学组成:100%氰丙基聚硅氧烷,所属极性:强极性. XE-60,化学组成:25%氰乙基、75%二甲基聚硅氧烷,所属极性:中极性,适用范围:酯、硝基化合物,对照牌号:DB-225、HP-225、CPSIL-43、RSL-500. FFAP,化学组成:聚乙二醇硝基苯改性,所属极性:极性,适用范围:酸、醇、醛、酯、酮、腈,对照牌号:SP-1000、OV-351、BP-21、HP-FFAP. PEG-20M,化学组成:聚乙二醇-20M,所属极性:极性,适用范围:酸、醇、醛、酯、甘醇,对照牌号:HP-20M、DB-WAX、007-20M、BP-20. LZP-930,化学组成:LZP,所属极性:极性,适用范围:白酒. AL2 O3,化学组成:r- AL2 O3,所属极性:极性,适用范围:C 1 -C 6 低适用范围:碳烃,对照牌号:Alumina

5A,化学组成:5A分子筛,所属极性:极性,适用范围:惰性气体及同位素.

C-2000,化学组成:碳分子筛,所属极性:极性,适用范围:He,H2 ,O2 ,CO,CO2, C1 -C2,对照牌号:CarbPLOT P 7.

13X,化学组成:13X分子筛,所属极性:极性,适用范围:石脑油 C3 -C12 环烷烃、链烷烃.

范文五：气相色谱中强极性柱、中极性柱、弱极性柱的区别

一、非极性

1、 100%Dimethyl polysiloxane, 100%二甲基聚硅氧烷,商品名:AC1, OV-101, OV-1, DB-1, SE-30, HP-1, RTX-1, BP-1

二、弱极性

2、 5%Phenyl dimethyl polysiloxane, 5%二苯基 (95%)二甲基聚硅氧烷,商品名:AC5, SE-52,

3、 5% Phenyl 1%vinyl dimethyl polysiloxane, 5%二苯基 1%乙烯基 (94%)二甲基聚硅氧烷, 商品名:OV-5, DB-5, SE-54, HP-5, RTX-5, BP-5

注:2、 3常无严格区分,通常混称。

三、中等极性

4、 50%Phenyl dimethyl polysiloxane, 50%二苯基 (50%)二甲基聚硅氧烷, 商品名:OV-17, HP-50, RTX-50

5、 14%Cyanopropyl phenyl polysiloxane, 14%氰丙基苯基 (其中 7%氰丙基 7%苯基 )(86%)二甲基聚硅氧烷,商品名:AC10, OV-1701, DB-1701, RTX-1701

6、 50% Cyanopropyl phenyl polysiloxane, 50%氰丙基苯基 (其中 25%氰丙基 25%苯基 )(50%)二甲基聚硅氧烷, 商品名:AC225, OV-225, BP-225, DB-225, HP-225, RTX-225

四、强极性

7、 polyethylene glycol,聚乙二醇,商品名:AC20, PEG20M , HP-INNOWAX(FFAP是其与 2-硝基对苯二甲酸的反应产物 ) 常用毛细管色谱柱对应表

SE-30、 OV-1,化学组成:100%甲基聚硅氧烷(胶体),所属极性:非极性,适用范围:碳氢化合物、农药、酚、胺, 对照牌号:DB-1、 BP-1、 007-1、 SPB-1 、 RSL-150、 CPSRL-5 、 HP-1.

OV-101, 化学组成:100%甲基聚硅氧烷(流体) , 所属极性:非极性 , 适用范围:氨基酸、碳氢化合物、药物胺 , 对照牌号:HP-100、 SP-2100

SE-52、 SE-54, 化学组成:5%苯基聚硅氧烷、 1%乙烯基 5%苯基甲基聚硅氧烷 , 所属极性:弱极性 , 适用范围:多核芳烃、酚、酯、碳氢化合物、药物胺 , 对照牌号:DB-5 、 BP-5、 SPB-5、 007-2 、 OV-73、 CPSIL-8、 RSL-120 、 HP-5.

OV-1701, 化学组成:7%氰丙基、 7%苯基甲基聚硅氧烷 , 所属极性:中极性 , 适用范围:药物、醇、酯、硝基苯类、除莠剂 , 对照牌号:BP-10、 RSL-1701、 DB-1701、 HP-1701、 CPISL-19. OV-17, 化学组成:50%苯基 50%甲基聚硅氧烷 , 所属极性:中极性 , 适用范围:药物、农药 , 对照牌号:DB-17、 HP-17、 007-17、 SP-2250、 RSL-300.

OV35, 化学组成:35%苯基 65%二甲基聚硅氧烷 , 所属极性:中极性 .

OV-225, 化学组成:25%氰丙基、 25%苯基、甲基聚硅氧烷 , 所属极性:中极性 , 适用范围:脂肪酸甲脂、碳水化合物、中行固醇 , 对照牌号:DB-225、 HP-225、 BP-225、 CPSIL-43、 RSL-500.

OV-275, 化学组成:100%氰丙基聚硅氧烷 , 所属极性:强极性 .

XE-60, 化学组成:25%氰乙基、 75%二甲基聚硅氧烷 , 所属极性:中极性 , 适用范围:酯、硝基化合物 , 对照牌号:DB-225、 HP-225、 CPSIL-43、 RSL-500.

FFAP, 化学组成:聚乙二醇硝基苯改性 , 所属极性:极性 , 适用范围:酸、醇、醛、酯、酮、腈 , 对照牌号:SP-1000、 OV-351、 BP-21、 HP-FFAP.

PEG-20M, 化学组成:聚乙二醇 -20M, 所属极性:极性 , 适用范围:酸、醇、醛、酯、甘醇 , 对照牌号:HP-20M 、 DB-WAX 、 007-20M 、 BP-20.

LZP-930, 化学组成:LZP, 所属极性:极性 , 适用范围:白酒 .

AL2 O3,化学组成:r- AL2 O3,所属极性:极性 , 适用范围:C 1 -C 6 低适用范围:碳烃 , 对照牌号:Alumina

5A, 化学组成:5A 分子筛 , 所属极性:极性 , 适用范围:惰性气体及同位素 .

C-2000, 化学组成:碳分子筛 , 所属极性:极性 , 适用范围:He,H2 ,O2 ,CO,CO2, C1 -C2,对照牌号:CarbPLOT P 7.

13X, 化学组成:13X 分子筛 , 所属极性:极性 , 适用范围:石脑油 C3 -C12 环烷烃、链烷烃 .

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